rabbit anti mouse rankl polyclonal antibody  (OriGene)

Journal: BMC Musculoskeletal Disorders

Article Title: High molecular weight hyaluronic acid alleviates ovariectomy-induced bone loss in mice

doi: 10.1186/s12891-024-08161-y

Figure Lengend Snippet: HMW-HA downregulated osteoclastic bone resorption in ovariectomized mice through the downregulation of RANKL-expressing osteocytes. A The area analyzed for osteoclast parameters is indicated in the box in yellow. B Representative images of TRAP staining. Low-power images boxed in red are enlarged and presented in each panel. Black arrows indicate osteoclasts. Red bars in high-power images indicate scale bars (50 μm). C Bone resorption parameters including osteoclast surface per bone surface (Oc.S/BS) and osteoclast number per bone surface (N.Oc/BS) of each sagittal section. D The area analyzed for Rankl + osteocyte percentage is indicated in the box in green. E Representative images of RANKL-positive osteocyte lacunes. Low-power images boxed in red are enlarged and presented in each panel. Black arrows indicate RANKL-positive osteocyte lacunes. Red arrows indicate osteocytes with no RANKL expression. Red bars in high-power images indicate scale bars (50 μm). F The Rankl. + osteocyte percentage was qualitatively assessed in each group. Data are expressed as mean ± SD, analyzed by ANOVA followed by Tukey’s post hoc test, showing all data points. * p < 0.05, ** p < 0.01, *** p < 0.001. The number of samples are indicated at the bottom of each figure and in Supplementary Tables 3 and 4

Article Snippet: Rabbit anti-mouse RANKL polyclonal antibody (cat#GTX32834) was purchased from Funakoshi (Tokyo, Japan).

Techniques: Expressing, Staining

Journal: BMC Musculoskeletal Disorders

Article Title: High molecular weight hyaluronic acid alleviates ovariectomy-induced bone loss in mice

doi: 10.1186/s12891-024-08161-y

Figure Lengend Snippet: Removal of HMW-HA from estrogen-deficient culture medium increased the RANKL expression in ST2 cells. A Experimental plan. Three hundred thousand cells were incubated for 12 h in RPMI-1640 medium. After cells were attached to the dishes, culture media were replaced with (I) charcoal-stripped FBS and (II) charcoal-stripped FBS with 100 nM of hyaluronidase for 12 h or 24 h. B , C Relative expression of RANKL and OPG in the presence or absence of hyaluronidase at 12 and 24 h. Colorful lines are associated with each 12 and 24 h samples. D Average ratio of RANKL mRNA to OPG mRNA in the presence or absence of hyaluronidase. Data are expressed as mean ± SD, analyzed by Wilcoxon signed-rank test ( B , C ), Wilcoxon rank-sum test ( D ), showing all data points. * p < 0.05. ns, not significant. n = 6 per group. Numerical data are presented in Supplementary Table 6

Article Snippet: Rabbit anti-mouse RANKL polyclonal antibody (cat#GTX32834) was purchased from Funakoshi (Tokyo, Japan).

Techniques: Expressing, Incubation

Journal: International journal of oncology

Article Title: Semaphorin 4D promotes bone invasion in head and neck squamous cell carcinoma.

doi: 10.3892/ijo.2017.4050

Figure Lengend Snippet: Figure 4. Effects of Sema4D on the formation and activity of osteoclasts in vitro. (A) BMMs were cultured with RANKL and M-CSF in the presence or absence of Sema4D. The number of TRAP-positive multinucleated cells in each well was counted. (B) BMMs were cultured on hydroxyapatite-coated plates. The area of pits on the plates was quantified by ImageJ. (C) ST-2 cells and primary mouse osteoblasts were cultured with Sema4D. RANKL expression was examined by immunoblot analysis. (D) BMMs were cultured without RANKL with M-CSF in the presence or absence of Sema4D (100 ng/ml) and OPG. The number of TRAP-positive multinucleated cells in each well was counted. All experiments were repeated three times. Data are the mean ± SD of triplicates from a typical experiment. *p<0.05.

Article Snippet: The membranes were blocked with 2% non-fat dry milk in TBS overnight at 4 ̊C and then incubated with rabbit anti-human Sema4D polyclonal IgG, rabbit anti-human Plexin-B1 polyclonal IgG (Abcam) or rabbit anti-mouse RANKL polyclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies used at a 1:200 dilution.

Techniques: Activity Assay, In Vitro, Cell Culture, Expressing, Western Blot

Journal: International journal of oncology

Article Title: Semaphorin 4D promotes bone invasion in head and neck squamous cell carcinoma.

doi: 10.3892/ijo.2017.4050

Figure Lengend Snippet: Figure 6. Role of Sema4D in bone invasion in HNSCC. IGF-I, which is released from bone, increases Sema4D secretion in HNSCC cells. Sema4D acts on HNSCC cells in an autocrine manner, which enhances cell activi- ties. Sema4D also regulated RANKL expression in osteoblasts stimulating osteoclastgenesis.

Article Snippet: The membranes were blocked with 2% non-fat dry milk in TBS overnight at 4 ̊C and then incubated with rabbit anti-human Sema4D polyclonal IgG, rabbit anti-human Plexin-B1 polyclonal IgG (Abcam) or rabbit anti-mouse RANKL polyclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies used at a 1:200 dilution.

Techniques: Expressing